recombinant mouse igf1 r d systems  (R&D Systems)

Journal: Neuron

Article Title: IGF1-dependent synaptic plasticity of mitral cells encodes olfactory memory during social learning

doi: 10.1016/j.neuron.2017.06.015

Figure Lengend Snippet: (A) IGF1 was applied to acute MOB slices while OS-M EPSCs (example traces, right) were recorded. (B, C) Summarized time course (B) and normalized amplitude analysis of OS-M EPSCs before and 15–25 mins after the initiation of IGF1 application (C) showing no significant effect of IGF1 on the strength of OS-M synaptic transmission. (D) Acute MOB slices treated with IGF1 with or without pre-incubation of selective IGF1-R kinase inhibitor NVP-AEW541 (1 μM) in ACSF while G-M IPSCs (example traces, right) were recorded. (E, F) Summarized time course (E) and normalized amplitude of G-M IPSCs before and 15–25 mins after the initiation of IGF1 treatment (F) showing the specificity of IGF1-induced synaptic potentiation. (G, H) Example mIPSC traces (G), averaged mIPSC frequency and amplitude (H) of mitral cells with (IGF1) or without (Ctrl) IGF1 treatment. (I, J) Representative traces (I) and analysis of averaged paired-pulse ratio (PPR) before and during IGF1 treatment (J). (K) Acute MOB slices with deletions of either IGF1-R or IR treated with IGF1 while G-M IPSCs (example traces, right) were recorded. (L, M) Summarized time courses (L) and normalized amplitude of G-M IPSCs before and 15–25 mins after the initiation of IGF1 treatment (M) showing the dominant role of IGF1-R versus IR in IGF1-induced synaptic potentiation. Data are means ± SEM (error bars), with cell number as sample size n. Numbers of neurons/mice are indicated in bars. Statistical analysis is by Student’s t test (**P < 0.01; *P < 0.05; n.s. P > 0.1).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Abcam Cat. No. ab290; RRID: AB_303395 Syt1 Synaptic System Cat. No. 105002; RRID: AB_887830 Syt10 NeuroMab Cat. No. 75-262; RRID: AB_10671950 vGlut1 Millipore Cat. No. AB5905; RRID: AB_2301751 vGAT Millipore Cat. No. AB5062P; RRID: AB_2301998 parvalbumin Millipore Cat. No. MAB1572; RRID: AB_11211313 IGF1-R Abcam Cat. No. ab131476; RRID: AB_11155487 GAPDH Cell Signaling Cat. No. 5174; RRID: AB_10622025 Mouse lines M72-GFP JAX Mice Stock No: 007766 M71-GFP JAX Mice Stock No: 006676 Syt10 f/f JAX Mice Stock No: 008413 IGF1-R f/f JAX Mice Stock No: 012251 IR f/f JAX Mice Stock No: 006955 Ai9 JAX Mice Stock No: 007905 PV-ires-Cre JAX Mice Stock No: 008069 Cdhr1-Cre MMRRC STOCK Tg(Cdhr1-cre)KG66Gsat/Mmucd Chemicals and peptide D-AP5 / CNQX Tocris Cat. No. 0106 / Cat. No. 0190 Picrotoxin Tocris Cat. No. 1128 TTX Tocris Cat. No. 1078 IGF1 R&D Systems Cat. No. 791-MG NVP-AEW541 Selleckchem Cat. No. S1034 AAV vectors AAV-Syt1 KD Stanford Vector Core NA AAV-GFP Stanford Vector Core NA AAV-Cre Stanford Vector Core NA AAV-DIO-GCaMP6m Stanford Vector Core NA Hardware Fiber photometry system ThinkerTech, Nanjing FIS Patch clamp system Molecular Devices MultiClamp 700B Confocal imaging system Olympus Inc. FV1200 Software Clampfit 10.4 Molecular Devices https://www.moleculardevices.com Image J NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques: Transmission Assay, Incubation

Journal: Neuron

Article Title: IGF1-dependent synaptic plasticity of mitral cells encodes olfactory memory during social learning

doi: 10.1016/j.neuron.2017.06.015

Figure Lengend Snippet: (A) Schematic diagram showing AAV injection, STFP training and subsequent slice recordings of G-M IPSCs from M72-associated mitral cells in M72-GFP + IGF1-R f/f mice. (B–D) Example G-M IPSC traces (left), summarized input-output curves (middle) and curve slopes (right) from M72-associated mitral cells in control (Ctrl) and IGF1-R KO (Cre) MOBs of M72-GFP + IGF1-R f/f observers of plain food (B), Oct-food (C) and Acp-food (D). For IGF1-R KO efficiency and basal G-M IPSCs data, see Figure S6 (I, J). Data are means ± SEM (error bars), with mouse number as sample size n. Numbers of neurons/mice are indicated in bars. Statistical analysis is by Student’s t test (**P < 0.01; *P < 0.05; n.s. P > 0.1).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Abcam Cat. No. ab290; RRID: AB_303395 Syt1 Synaptic System Cat. No. 105002; RRID: AB_887830 Syt10 NeuroMab Cat. No. 75-262; RRID: AB_10671950 vGlut1 Millipore Cat. No. AB5905; RRID: AB_2301751 vGAT Millipore Cat. No. AB5062P; RRID: AB_2301998 parvalbumin Millipore Cat. No. MAB1572; RRID: AB_11211313 IGF1-R Abcam Cat. No. ab131476; RRID: AB_11155487 GAPDH Cell Signaling Cat. No. 5174; RRID: AB_10622025 Mouse lines M72-GFP JAX Mice Stock No: 007766 M71-GFP JAX Mice Stock No: 006676 Syt10 f/f JAX Mice Stock No: 008413 IGF1-R f/f JAX Mice Stock No: 012251 IR f/f JAX Mice Stock No: 006955 Ai9 JAX Mice Stock No: 007905 PV-ires-Cre JAX Mice Stock No: 008069 Cdhr1-Cre MMRRC STOCK Tg(Cdhr1-cre)KG66Gsat/Mmucd Chemicals and peptide D-AP5 / CNQX Tocris Cat. No. 0106 / Cat. No. 0190 Picrotoxin Tocris Cat. No. 1128 TTX Tocris Cat. No. 1078 IGF1 R&D Systems Cat. No. 791-MG NVP-AEW541 Selleckchem Cat. No. S1034 AAV vectors AAV-Syt1 KD Stanford Vector Core NA AAV-GFP Stanford Vector Core NA AAV-Cre Stanford Vector Core NA AAV-DIO-GCaMP6m Stanford Vector Core NA Hardware Fiber photometry system ThinkerTech, Nanjing FIS Patch clamp system Molecular Devices MultiClamp 700B Confocal imaging system Olympus Inc. FV1200 Software Clampfit 10.4 Molecular Devices https://www.moleculardevices.com Image J NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques: Injection, Control

Journal: Neuron

Article Title: IGF1-dependent synaptic plasticity of mitral cells encodes olfactory memory during social learning

doi: 10.1016/j.neuron.2017.06.015

Figure Lengend Snippet: (A) Schematic diagram showing AAV injection, food deprivation and subsequent food-finding test. (B–D) Analyses of time spent in finding buried food by the adult mice with Syt1 KD (B), Syt10 KO (C) and IGF1-R KO (D) in the MOB. (E) Schematic diagram showing AAV injection, habituation, STFP training and memory tests after STFP. (F–H) Analyses of memory retrieval for Acp-flavored food at different time points (immediately, 1 day and 14 days) after STFP by adult mice with intrabulbar Syt1 KD (F), Syt10 KO (G) and IGF1-R KO (H). For other behavioral tests, see Figure S7. Data (B–D, F–H) are means ± SEM (error bars), with mouse number as sample size n. Numbers of mice are indicated in bars. Statistical analysis is by Student’s t test (***P < 0.001; **P < 0.01; *P < 0.05; n.s. P > 0.1).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Abcam Cat. No. ab290; RRID: AB_303395 Syt1 Synaptic System Cat. No. 105002; RRID: AB_887830 Syt10 NeuroMab Cat. No. 75-262; RRID: AB_10671950 vGlut1 Millipore Cat. No. AB5905; RRID: AB_2301751 vGAT Millipore Cat. No. AB5062P; RRID: AB_2301998 parvalbumin Millipore Cat. No. MAB1572; RRID: AB_11211313 IGF1-R Abcam Cat. No. ab131476; RRID: AB_11155487 GAPDH Cell Signaling Cat. No. 5174; RRID: AB_10622025 Mouse lines M72-GFP JAX Mice Stock No: 007766 M71-GFP JAX Mice Stock No: 006676 Syt10 f/f JAX Mice Stock No: 008413 IGF1-R f/f JAX Mice Stock No: 012251 IR f/f JAX Mice Stock No: 006955 Ai9 JAX Mice Stock No: 007905 PV-ires-Cre JAX Mice Stock No: 008069 Cdhr1-Cre MMRRC STOCK Tg(Cdhr1-cre)KG66Gsat/Mmucd Chemicals and peptide D-AP5 / CNQX Tocris Cat. No. 0106 / Cat. No. 0190 Picrotoxin Tocris Cat. No. 1128 TTX Tocris Cat. No. 1078 IGF1 R&D Systems Cat. No. 791-MG NVP-AEW541 Selleckchem Cat. No. S1034 AAV vectors AAV-Syt1 KD Stanford Vector Core NA AAV-GFP Stanford Vector Core NA AAV-Cre Stanford Vector Core NA AAV-DIO-GCaMP6m Stanford Vector Core NA Hardware Fiber photometry system ThinkerTech, Nanjing FIS Patch clamp system Molecular Devices MultiClamp 700B Confocal imaging system Olympus Inc. FV1200 Software Clampfit 10.4 Molecular Devices https://www.moleculardevices.com Image J NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques: Injection

Journal: Neuron

Article Title: IGF1-dependent synaptic plasticity of mitral cells encodes olfactory memory during social learning

doi: 10.1016/j.neuron.2017.06.015

Figure Lengend Snippet: (A) Odor exposure without a social context induces transient Ca2+ entry that triggers Syt1-dependent glutamate release but not Syt10-dependent IGF1 secretion from mitral cell dendrite. Such process then evokes GABA release from granule cell and feedback inhibition on the mitral cell without G-M synaptic potentiation. (B) Odor exposure in a social context during STFP induces sustained Ca2+ entry in mitral cell dendrites and triggers both glutamate release and IGF1 exocytosis. IGF1 acts on IGF1-R and potentiates G-M synaptic strength by recruiting more GABA-R, which may be a synaptic substrate for the association of odor signals and social context.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Abcam Cat. No. ab290; RRID: AB_303395 Syt1 Synaptic System Cat. No. 105002; RRID: AB_887830 Syt10 NeuroMab Cat. No. 75-262; RRID: AB_10671950 vGlut1 Millipore Cat. No. AB5905; RRID: AB_2301751 vGAT Millipore Cat. No. AB5062P; RRID: AB_2301998 parvalbumin Millipore Cat. No. MAB1572; RRID: AB_11211313 IGF1-R Abcam Cat. No. ab131476; RRID: AB_11155487 GAPDH Cell Signaling Cat. No. 5174; RRID: AB_10622025 Mouse lines M72-GFP JAX Mice Stock No: 007766 M71-GFP JAX Mice Stock No: 006676 Syt10 f/f JAX Mice Stock No: 008413 IGF1-R f/f JAX Mice Stock No: 012251 IR f/f JAX Mice Stock No: 006955 Ai9 JAX Mice Stock No: 007905 PV-ires-Cre JAX Mice Stock No: 008069 Cdhr1-Cre MMRRC STOCK Tg(Cdhr1-cre)KG66Gsat/Mmucd Chemicals and peptide D-AP5 / CNQX Tocris Cat. No. 0106 / Cat. No. 0190 Picrotoxin Tocris Cat. No. 1128 TTX Tocris Cat. No. 1078 IGF1 R&D Systems Cat. No. 791-MG NVP-AEW541 Selleckchem Cat. No. S1034 AAV vectors AAV-Syt1 KD Stanford Vector Core NA AAV-GFP Stanford Vector Core NA AAV-Cre Stanford Vector Core NA AAV-DIO-GCaMP6m Stanford Vector Core NA Hardware Fiber photometry system ThinkerTech, Nanjing FIS Patch clamp system Molecular Devices MultiClamp 700B Confocal imaging system Olympus Inc. FV1200 Software Clampfit 10.4 Molecular Devices https://www.moleculardevices.com Image J NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques: Inhibition

Journal: Neuron

Article Title: IGF1-dependent synaptic plasticity of mitral cells encodes olfactory memory during social learning

doi: 10.1016/j.neuron.2017.06.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Abcam Cat. No. ab290; RRID: AB_303395 Syt1 Synaptic System Cat. No. 105002; RRID: AB_887830 Syt10 NeuroMab Cat. No. 75-262; RRID: AB_10671950 vGlut1 Millipore Cat. No. AB5905; RRID: AB_2301751 vGAT Millipore Cat. No. AB5062P; RRID: AB_2301998 parvalbumin Millipore Cat. No. MAB1572; RRID: AB_11211313 IGF1-R Abcam Cat. No. ab131476; RRID: AB_11155487 GAPDH Cell Signaling Cat. No. 5174; RRID: AB_10622025 Mouse lines M72-GFP JAX Mice Stock No: 007766 M71-GFP JAX Mice Stock No: 006676 Syt10 f/f JAX Mice Stock No: 008413 IGF1-R f/f JAX Mice Stock No: 012251 IR f/f JAX Mice Stock No: 006955 Ai9 JAX Mice Stock No: 007905 PV-ires-Cre JAX Mice Stock No: 008069 Cdhr1-Cre MMRRC STOCK Tg(Cdhr1-cre)KG66Gsat/Mmucd Chemicals and peptide D-AP5 / CNQX Tocris Cat. No. 0106 / Cat. No. 0190 Picrotoxin Tocris Cat. No. 1128 TTX Tocris Cat. No. 1078 IGF1 R&D Systems Cat. No. 791-MG NVP-AEW541 Selleckchem Cat. No. S1034 AAV vectors AAV-Syt1 KD Stanford Vector Core NA AAV-GFP Stanford Vector Core NA AAV-Cre Stanford Vector Core NA AAV-DIO-GCaMP6m Stanford Vector Core NA Hardware Fiber photometry system ThinkerTech, Nanjing FIS Patch clamp system Molecular Devices MultiClamp 700B Confocal imaging system Olympus Inc. FV1200 Software Clampfit 10.4 Molecular Devices https://www.moleculardevices.com Image J NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques: Plasmid Preparation, Patch Clamp, Imaging, Software